NATIONAL CENTRE FOR LABORATORY ANIMAL SCIENCES (NCLAS)

SOME OF THE BASIC REQUIREMENTS

I. Environmental Conditions

Temperature                                 : 25?C ± 2?C throughout the year.
Relative humidity                           : 45-55% throughout the year.
Ventilation(number of air changes) : 16-20 fresh air changes/hour (Recirculation of air from animal rooms is not recommended).
Light-dark cycle                            : 12 hours light and 12 hours dark or 14 hours light and 10 hours dark.
Light intensity                               : 350-400 lux one metre above the floor level.
Light source                                : Fluorescent tube lights fixed in such a way to facilitate uniform distribution of light.
Noise level                                   : Should be less than 65 decibels (should avoid all high frequency sound).

II. Hygiene and Care

A high degree of hygienic conditions should be maintained. Periodical cleaning and sterilisation of cages, diet and water containers and cleaning and disinfection of rooms should be ensured. Animal rooms should be rodent and other vector proof Pest control measures should be undertaken periodically. Use of any pesticide that may have deleterious effect on animals should be avoided. "Pyrethrum", a plant extract can be safely used as a pesticide in animal rooms. Trained personnel should undertake the day-to-day care of the animals.

III. Nutritional Requirements

The animal should be provided with clean feed and water free of pathogenic organisms. The feed should meet the nutritional requirements of the animals. Water should be free of all pathogenic organisms. Boiled, cooled water can be used. Acidification of water with conc. HCL to adjust the pH to 2.8 to 3.2 is good (Add about 3 ml of HCL to 1 litre and boiled cooled water).

IV. Ethical Considerations and Experimental Protocols

Absolute care should be taken to avoid inflicting unnecessary and avoidable pain to the animals. Experimental procedures on the animals should be carried out only under the supervision/guidance of appropriately qualified persons. An Ethical Committee consisting of experts should approve experiments involving live animals. While planning any experiment it is recommended that you may consult a statistician for determining the minimum sample size. The pricing of the animals is done on the basis of various inputs. It will work out cheaper (and quality assured) to buy animals from our facility rather than attempting to breed the animals in the user's premises. The facility has the capacity and potential to breed and supply large number of different species and strains of laboratory animals as per customer's requirement. Details of the species and strains of animals available for supply and present price and terms and conditions of supply, and ordering procedures are furnished for the convenience of prospective buyers.

HOW TO ORDER THE ANIMALS

1. While ordering please specify the following:

(a) Your CPCSEA registration Number
(b) Your Institutional Animal Ethics Committee's approval for the animals required
(c) Species
(d) Strain
(e) Age and /or weight range
(f) No. of animals required
(g) Sex (orders for both male and females will be given preference)
(h) Date on which the animals are required
(i) Nearest airport
(j) Distance from the airport to the Institute
(k) Telegraphic code, Fax number and Telephone numbers
(l) Residence Telephone number of the officer ordering for the animals.
    (this will facilitate communicating despatch details)


    Although orders can be executed at short notice for small numbers of animals, it is desirable to give advance notice of 30-40 days regarding requirement for animals. This will ensure better planning of the breeding programme and supply of animals according to the specifications and on the date required.

2. Animals are generally supplied against advance payment either in cash or by demand draft payable to the Director NCLAS at the branches of the Nationalised Banks in Secunderabad. Outstation cheques will not be accepted.

3. Animals can be either collected from our Animal Facility or air freighted to the nearest airport. In case of air-freighting, packing, forwarding and handling charges will be extra. Animals will not be sent unaccompanied by rail.

4. Appropriate kind of transport boxes for air freighting animals are available from the facility. Those who come to collect animals from our facility, please ensure to bring appropriate size and adequate number of cages.

5. Prompt acknowledgement of receipts of animals by air is requested

6. As soon as the animals are received please transfer them to clean cages and feed clean water. Transportation is a stress on animals. Therefore it is advisable to use the animals for experimentation or breeding only after an acclimatization period of 7 to 15 days in the new environment.

7. We will not be responsible for any death, injury or escape of animals after they leave our premises. Requests for free replacement of animals or refund of cost will not be entertained.

8. Feed-back on the quality of animals supplied by us in terms of experimental results will be appreciated. It will enable us to monitor the quality of animals.

9. Indents for animals may please be sent to:

    Officer-in-charge
    National Centre for Laboratory Animal Sciences
    National Institute of Nutrition
    Jamai Osmania
    Hyderabad - 500 007.
    Telegram : NUTRITION
    Fax : 040-27003317 / 27019074
    Telephone
    Monday to Friday between 9.00 am. & 5.30 pm 27197201/202/205/207
    After office hours and on holidays : 27197200
    Residence: Dr.N.V Giridharan: 27016258/09885442373; Dr.P Suresh: 09849178671
    Email, nappanveettil@yahoo.co.in, sureshpothani@gmail.com;
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NATIONAL CENTRE FOR LABORATORY ANIMAL SCIENCES NATIONAL INSTITUTE OF NUTRITION HYDERABAD (W.E.F 1st AUGUST 2009) PRICE LIST OF ANIMALS, FEEDS, BLOOD AND BLOOD PRODUCTS (In Rupees)

Species Strain /breed Govt, Non-profit making Organisations Private, Profit making Organisations.     Weaning Post-Weaning Retired Weaning Post-Weaning retired Mouse Swiss (I.B) 45.00 80.00 45.00 60.00 110.00 60.00   BALB/C An.N (I.B) 45.00 80.00 45.00 60.00 110.00 60.00   C57 BL/6J (I.B) 45.00 80.00 45.00 60.00 110.00 60.00   FVB/N(I.B) 45.00 80.00 45.00 60.00 110.00 60.00 Rat Wistar/NIN (I.B) 80.00 160.00 80.00 120.00 220.00 120.00   CFY/NIN (I.B) 80.00 160.00 80.00 120.00 220.00 120.00   Fischer 344 N (I.B) 80.00 160.00 80.00 120.00 220.00 120.00   Sprague Dawley (O.B) 80.00 160.00 80.00 120.00 220.00 120.00   Holtzman (O.B) 80.00 160.00 80.00 120.00 220.00 120.00   Wistar Kyoto (I.B) 80.00 160.00 80.00 120.00 220.00 120.00 Guinea Pig NIH: Hartly (O.B)   440.00     550.00     NIH: Coloured (O.B) 440.00 550.00 Rabbit New Zeeland (O.B) 1540.00 1925.00 Hamster Golden (Syrian)(I.B) 80.00 120.00 60.00 120.00 165.00 120.00                                                                                       Animal Feeds 20% Protein (Rat, Mouse & Hamster) 95.00 110.00 14% Protein (Rabbit, Guinea Pigs & Monkey) 70.00 90.00     Species   Strain /breed Positive Pressure Isolator Bred SPF (Animal Breeding Nuclei, Price common for Govt. & Private)   *Pedigreed animals for Breeding   *Timed Pregnant animals     Weaning Post-Weaning Government Private Government Private Mouse Swiss (I.B)   145.00     180.00 160.00 205.00   BALB/C An.N (I.B) 155.00 190.00 145.00     180.00 160.00 205.00   C57 BL/6J (I.B)   145.00     180.00 160.00 205.00 N:NIH(S) NUDE (ATHYMIC) Heterozygous male 155.00 190.00 * Subject to availability and limited numbers only Heterozygous female 275 .00 390.00 Homozygous male 275.00 390.00 Homozygous female 155.00 190.00 Rat Wistar/NIN (I.B) 190.00 245.00 220.00 300.00 240.00 300.00 Sprague Dawley(O.B) 190.00 245.00 220.00 300.00 240.00 300.00 Fischer 344 (I.B)                      220.00 300.00 240.00 300.00 Wistar Kyoto       220.00 300.00 240.00 300.00 CFY/NIN (I.B) 220.00 300.00 240.00 300.00 Holtzman (O.B) 220.00 300.00 240.00 300.00 Hamster ( Syrian )           155.00 270.00 Lactating Mouse with litter                                200.00 270.00 Lactating rat with litter         400.00 600.00 Lactating Hamster With litter         245.00 400.00 Note:-  (a) I.B : Inbred; O.B : Out bred;  (b) Weaning: Rat & Mouse 21 - 24 days age. (c) Packing and forwarding charges will be extra. Handling charges to meet the cost of telecommunication, transport etc.,a sum of Rs. 250.00 will also be charged for each consignment. Details of Transport boxes Available Type Dimensions Volumetric weight Max. No. of animals accommodated Price Small 27 x 23 x 18 cms 1.5 kg Adult Mice 25 Weaning Rats 10 120.00 Mouse 55 x 30 x 15 cms 4.1 kg Adult 50 Hamsters 10 - 15 240.00 Rat 55 x 30 x 20 cms 5.5 kg Weaning 20 Adult  10 240.00 G.Pig 65 x 35 x 20 cms 7.5 kg 150 - 250 g 15 >250g 10 300.00 Rabbit 65 x 45 x 25 cms 12.5 kg Adult 5 450.00 These transport boxes are meant for only carrying animals and can be reused after sterilisation.  Animals should not be housed in these boxes for more than 24 hours. Blood and Sera (Rupees)              Species Blood (ml) Plasma/Sera (ml)   Government Private Government Private Mouse 55.00 115.00 115.00 230.00 Rat 35.00 70.00 70.00 135.00 Hamster 50.00 100.00 100.00 200.00 Guinea Pig 70.00 135.00 135.00 265.00 Rabbit 50.00 100.00 100.00 200.00 Monkey 50.00 100.00 100.00 200.00 Sheep/Goat 25.00 45.00 45.00 90.00 Note:    Packing and forwarding charges (Min. Rs. 300.00) and Handling charges Rs.250.00 extra the cost of the material.   Maintenance Charges for conducting experiments at NCLAS (Per day per animal & in Rs.) Species Government Private Mouse 5.00 10.00 Rat 8.00 15.00 Hamster 8.00 15.00 Guinea Pig 20.00 35.00 Rabbit 55.00 85.00 Monkey 90.00 110.00 Animal leasing charges Rs. 500.00 / monkey / day extra. HEALTH MONITORING 1. Microbiology / Virology / sample          :  Rs.450.00   TISSUES / ORGANS Cost of Organs will be estimated on the basis of the following       1.Cost of animals.       2. Salary of the staff involved and number of hours worked.       3. Consumables and others Rs. 100 per animal.       4. Disposables PPE (for staff involved) Rs.100 per person.       5. 10% of total above will be administrative charges.